• br Results br Discussion Here we have shown that

  2018-10-22

  

  Results Discussion Here, we have shown that GATA4, MEF2C, TBX5, ESRRG, MESP1, MYOCD, and ZFPM2 together reprogram human fibroblasts toward a CM-like state. Human iCMs upregulated hundreds of CM-enriched genes, downregulated hundreds of fibroblast-enriched transcripts, and assembled sarcomeres.

• The higher methylation levels of non

  2018-10-22

  

  The higher methylation levels of non-CpG sites in human oocytes is consistent with what has been reported for other cell types that possess high potential for maturation or further differentiation, including mammalian kinesin inhibitor cell types and human pluripotent stem cells (Lister et al., 201

• g-quadruplex The following are the supplementary data relate

  2018-10-22

  

  The following are the supplementary data related to this article. Disclosure of conflict of interest Acknowledgments We thank Martin Rŭtze for expert help during manuscript revision, Nicole Buchta and Eva-Maria Maurer for technical assistance and the Robert-Mathys Foundation for the donation

• H-Lys(Ac)-OH.HCl The second most stable reference gene in t

  2018-10-22

  

  The second most stable reference gene in the present study comparing OA- and healthy BMSCs is IPO8. The IPO8 gene product is a member of the importin-β family responsible for the nuclear import of proteins (Dean et al., 2001). Furthermore, it is involved in cytoplasmic miRNA-guided gene silencing an

• A recent study showed that mesenchymal ALK regulates the bal

  2018-10-20

  

  A recent study showed that mesenchymal ALK5 regulates the balance between smooth muscle versus lipofibroblast differentiation and that ALK5 inhibition shifts the balance towards lipofibroblast (Li et al., 2014). Thus, it is possible that the enhancing effect of ALK5-I we observed in our culture syst

• br Materials and methods br

  2018-10-20

  

  Materials and methods Author disclosure statement Acknowledgments This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” (FIS PI10/0703 and PI13/00556 to RG and PI

• We recently reported that iPSCs can

  2018-10-20

  

  We recently reported that iPSCs can be generated using non-modified mRNA (Arnold et al., 2012). In this report we will focus on the crucial steps for successfully applying this method. Material and methods Results Discussion Acknowledgments The work was supported by funding from the Ger

• Dig-11-ddUTP To confirm the pluripotency of the MIXL eGFP hE

  2018-10-20

  

  To confirm the pluripotency of the MIXL1-eGFP hESCs, the expression of several pluripotency markers was analyzed by quantitative real-time PCR as well as immunocytochemsitry. Endogenous expression of NANOG, OCT4 and SOX2 was determined at the mRNA level by real-time PCR (Fig. 1C). Protein expression

• br Materials and methods br Acknowledgements This study

  2018-10-20

  

  Materials and methods Acknowledgements This study was supported by the European Union within the 7th European Community Framework Programme through funding for the NEUROMICS network (F5-2012-305121 to L.S.), the Marie Curie International Outgoing Fellowship (grant PIOF-GA-2012-326681 to R.S.

• The four cell lines present the typical morphology

  2018-10-20

  

  The four cell lines present the typical morphology of pluripotent stem cells, growing in compact colonies, with tightly packed mao inhibitor (Fig. 1B). They express the pluripotency markers SSEA4, SSEA3, TRA-1-81, TRA-1-60 and OCT3/4, assessed by flow cytometry (Fig. 2A), and OCT4, REX1, NANOG and

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction The vital processes of blood coagulation, clot formation, and hemostasis rely upon a sufficient supply of platelets within a person’s bloodstream. Transfusion remains the most effective way to increase a patient’s

• microRNAs miRNAs are small noncoding RNAs of nucleotides tha

  2018-10-20

  

  microRNAs (miRNAs) are small noncoding RNAs of 22 nucleotides that control gene expression chiefly by translational repression (Lee et al., 2004). miRNA expression is spatially and temporally regulated during development, and they can participate in the regulation of pluripotent stem cell lineage co

• Although WNT signaling plays a prominent role in A

  2018-10-20

  

  Although WNT signaling plays a prominent role in A/P patterning of the neural tube, few studies have extensively examined the influence of WNT on the A/P positional identity of hPSC-derived NPCs and neurons. To date, most studies have relied on FGF8 (Yan et al., 2005; Yang et al., 2008) or retinoic

• buy RG7112 Evidence suggests that sex steroids play at

  2018-10-20

  

  Evidence suggests that sex steroids play at least some role in age-related degeneration of lymphopoiesis (Chinn et al., 2012), and we, and others, have previously shown that sex steroid ablation (SSA) is able to rejuvenate aged and immunodepleted BM and thymus, enhance peripheral T and B cell functi

• Partial cleavage of CXCL at

  2018-10-20

  

  Partial cleavage of CXCL12 at the N-terminus by distinct peptidases results in loss of chemotactic activity and impairment in CXCR4 receptor affinity (Cho et al., 2010; Delgado et al., 2001; Levesque et al., 2003). The proteolytic cleavage of CXCL12 by matrix metalloproteinase 2 and 9 (MMP-2 and 9)

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