• A recent study showed that mesenchymal ALK regulates the bal

  2018-10-20

  

  A recent study showed that mesenchymal ALK5 regulates the balance between smooth muscle versus lipofibroblast differentiation and that ALK5 inhibition shifts the balance towards lipofibroblast (Li et al., 2014). Thus, it is possible that the enhancing effect of ALK5-I we observed in our culture syst

• br Materials and methods br

  2018-10-20

  

  Materials and methods Author disclosure statement Acknowledgments This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” (FIS PI10/0703 and PI13/00556 to RG and PI

• We recently reported that iPSCs can

  2018-10-20

  

  We recently reported that iPSCs can be generated using non-modified mRNA (Arnold et al., 2012). In this report we will focus on the crucial steps for successfully applying this method. Material and methods Results Discussion Acknowledgments The work was supported by funding from the Ger

• Dig-11-ddUTP To confirm the pluripotency of the MIXL eGFP hE

  2018-10-20

  

  To confirm the pluripotency of the MIXL1-eGFP hESCs, the expression of several pluripotency markers was analyzed by quantitative real-time PCR as well as immunocytochemsitry. Endogenous expression of NANOG, OCT4 and SOX2 was determined at the mRNA level by real-time PCR (Fig. 1C). Protein expression

• br Materials and methods br Acknowledgements This study

  2018-10-20

  

  Materials and methods Acknowledgements This study was supported by the European Union within the 7th European Community Framework Programme through funding for the NEUROMICS network (F5-2012-305121 to L.S.), the Marie Curie International Outgoing Fellowship (grant PIOF-GA-2012-326681 to R.S.

• The four cell lines present the typical morphology

  2018-10-20

  

  The four cell lines present the typical morphology of pluripotent stem cells, growing in compact colonies, with tightly packed mao inhibitor (Fig. 1B). They express the pluripotency markers SSEA4, SSEA3, TRA-1-81, TRA-1-60 and OCT3/4, assessed by flow cytometry (Fig. 2A), and OCT4, REX1, NANOG and

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction The vital processes of blood coagulation, clot formation, and hemostasis rely upon a sufficient supply of platelets within a person’s bloodstream. Transfusion remains the most effective way to increase a patient’s

• microRNAs miRNAs are small noncoding RNAs of nucleotides tha

  2018-10-20

  

  microRNAs (miRNAs) are small noncoding RNAs of 22 nucleotides that control gene expression chiefly by translational repression (Lee et al., 2004). miRNA expression is spatially and temporally regulated during development, and they can participate in the regulation of pluripotent stem cell lineage co

• Although WNT signaling plays a prominent role in A

  2018-10-20

  

  Although WNT signaling plays a prominent role in A/P patterning of the neural tube, few studies have extensively examined the influence of WNT on the A/P positional identity of hPSC-derived NPCs and neurons. To date, most studies have relied on FGF8 (Yan et al., 2005; Yang et al., 2008) or retinoic

• buy RG7112 Evidence suggests that sex steroids play at

  2018-10-20

  

  Evidence suggests that sex steroids play at least some role in age-related degeneration of lymphopoiesis (Chinn et al., 2012), and we, and others, have previously shown that sex steroid ablation (SSA) is able to rejuvenate aged and immunodepleted BM and thymus, enhance peripheral T and B cell functi

• Partial cleavage of CXCL at

  2018-10-20

  

  Partial cleavage of CXCL12 at the N-terminus by distinct peptidases results in loss of chemotactic activity and impairment in CXCR4 receptor affinity (Cho et al., 2010; Delgado et al., 2001; Levesque et al., 2003). The proteolytic cleavage of CXCL12 by matrix metalloproteinase 2 and 9 (MMP-2 and 9)

• The activity of CXCL through CXCR receptor has been

  2018-10-20

  

  The activity of CXCL12(5-67) through CXCR3 receptor has been shown before (Denoyer et al., 2012; Zhu et al., 2009). Here we aimed to confirm this finding using CXCL9, a natural ligand of CXCR3. NSCs pre-treated with CXCL9 were not susceptible to the CXCL12(5-67) apoptotic effects. The effect of CXCL

• This study indicates that miR and miR may also be

  2018-10-20

  

  This study indicates that miR-124 and miR-219 may also be involved in the neurogenesis-to-gliogenesis switch by NSPCs. miR-124 promotes neurogenesis via downregulation of several genes (Sox9, Scp1, and Ezh2) that are positive regulators of gliogenesis or negative regulators of neurogenesis in the de

• Our initial endeavors in characterizing our isogenic

  2018-10-20

  

  Our initial endeavors in characterizing our isogenic iPSCs lines included a microarray-based large-scale gene Apremilast analysis comparing the HD iPSCs with the corrected line C116 iPSCs (An et al., 2012). These studies were restricted to a comparison of only the iPSCs lines but yielded several use

• To elucidate whether the expression pattern

  2018-10-20

  

  To elucidate whether the expression pattern of Zscan4 has any correlation with ESC proliferation, we monitored Zscan4 activity at single cell level. Also to see whether the rejuvenation factor Zscan4 correlates with the fluctuating wave of ESC pluripotency (Figure S1), we monitored Zscan4 and the pl

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